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KMID : 1234920140140010013
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Journal of Korean Oriental Association for Study of Obesity
2014 Volume.14 No. 1 p.13 ~ p.23
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Screening of Herbal Medicines for Synergistic Effects of Metformin and Herbal Extracts Combination in RAW 264.7 Cells
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Kim Hyung-Gu
Wang Jing-Hua
Chae Hee-Sung
Chin Young-Won
Choi Han-Seok
Kim Ho-Jun
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Abstract
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Objectives: This study was performed to evaluate the effects of Metformin and Lonicerae Flos, Agrobacterium Rhizogenes, Coptidis Rhizoma, Atractylodis Rhizoma Alba, Houttuyniae Herba extracs combinations on hypoglycemia in RAW 264.7 cells.
Methods: Expressions of Sirt1, p-adenosine monophosphate-activated kinase (p-AMPK), AMPKalpha, peroxisome proliferator activated receptor (PPAR)-alpha, PPAR-gamma, X-box binding protein 1 (XBP-1), tumor necrosis factor (TNF)-alpha and interleukin (IL)-6 were analyzed by real time polymerase chain reaction and Western blotting analysis.
Results: The level of gene expression of Sirt1, p-AMPK, AMPK-alpha, PPAR-alpha and XBP-1 in relation to that of beta-actin were increased or decreased significantly with the Metformin and Lonicerae Flos, Agrobacterium Rhizogenes extracts combination groups. The level of gene expression of TNF-alpha and IL-6 were increased significantly with the Metformin and Houttuyniae Herba, Coptidis Rhizoma extracts combination groups.
Conclusions: Metformin and Lonicerae Flos, Agrobacterium Rhizogenes extracts combination groups showed synergistic hypoglycemic effects by increasing AMPK and PPAR gene expression in RAW 264.7 cells.
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KEYWORD
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Metformin, Herbal medicines, Synergistic effects, Combination, Hypoglycemia
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